Reporter
GFP

Part:BBa_K845002:Design

Designed by: Grégory Hansen   Group: iGEM12_Grenoble   (2012-09-26)

Strong RBS GFPmut2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670


Design Notes

Our safety note: https://static.igem.org/mediawiki/2012/2/2a/ParaBAD-gfp.pdf

Source

The GFP protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence.

References

Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., Liebermeister, W., et al (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods, 3(8), 623-628. doi:10.1038/nmeth895 ( http://www.ncbi.nlm.nih.gov/pubmed/16862137 )